【摘 要】
：
One parasitic fungus strain PLHN was isolated from female of Meloidogyne sp.,which damaged to eggplant in Hainan province.Cultural and morphological characteristics of PLHN were examined,its rDNA ITS
【机 构】
：
College of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China
论文部分内容阅读
One parasitic fungus strain PLHN was isolated from female of Meloidogyne sp.,which damaged to eggplant in Hainan province.Cultural and morphological characteristics of PLHN were examined,its rDNA ITS gene and serine protease gene sequence were cloned and analyzed,and its biological characteristics were determined.The results showed as follows: the color of strain PLHN colony on potato dextrose ager (PDA) plate was pale purple,and powdery conidia were produced abundantly on the colony.The back of colony was light beige.Phialides developed at the end of the conidiophore were in whorls of 2 ~ 6 and in size of (2.81 ± 0.43) μm × (6.27 ± 1.18) μm;Conidia were subrotund,colorless and in size of (2.60± 0.40) μm × (2.63 ± 0.38) μm.The sequences of rDNA ITS and serine protease gene of strain PLHN shared 99% ~ 100% similarity with corresponding sequences of some Purpureocillium lilacinum stranis (primitive named Paecilomyces lilacinus) deposited in GenBank.According to its morphological characteristics and molecular biology,strain PLHN was identified as P.lilacinum.The optimal medium was potato dextrose agar (PDA).The optimal pH and temperature for mycelia growth,sporulation and conidial germination were 7.0 and 28℃ respectively.The lethal temperature to conidium was 55℃.And darkness was beneficial to PLHN.
其他文献
食细菌线虫是土壤线虫主要的营养类群之一,其数量丰富、种类多样.细菌是土壤中最丰富的微生物,食细菌线虫与细菌通过捕食关系构成的食物链或微食物网是土壤食物网中重要的一部分,对土壤的物质循环和能量流动起关键的作用.文章概述了食细菌线虫捕食对土壤细菌和功能类群细菌丰度、活性及群落结构的影响,阐述了食细菌线虫与细菌互作对土壤功能的影响,并进一步阐述了食细菌线虫影响土壤细菌群落结构和活性的机理,为揭示土壤生物
植物寄生线虫是农业生产中常见的病原物,其中孢囊线虫和根结线虫造成的危害最为严重.目前在植物中已经克隆了一些与孢囊线虫和根结线虫抗性相关的基因,对其中部分基因作用机制的研究揭示了植物对病原物防卫反应的新机制.本文总结了植物中已克隆的与线虫抗性相关的基因,并以番茄抗根结线虫基因Mi和大豆抗孢囊线虫的两个主效基因rhg1和Rhg4为例,简要阐述了其作用机制.
分子标记技术由于能够反映DNA水平上的遗传变异,同时不受环境影响,且稳定、简便、精确,目前已经成为研究遗传多样性的重要方法.本研究简述了分子标记技术在线虫鉴定研究中的应用及遗传多样性研究中几种常用的分子标记技术,最后对分子标记用于马铃薯线虫的鉴定及遗传多样性研究进行了展望.
Root-knot nematodes are phytoparasites that trigger a long-lasting and intimate relationship with their host plants.They infect roots and establish specialized feeding structures called giant-cells,fr
Populations of Criconemoides myungsugae and C.parvus were isolated in Zhejiang Province China from the rhizosphere soil of Altingia gracilipes and Punica granatum.Both species are characterized molecu
The systematics and diagnostics of Longidorid nematodes are important because of regulatory and management issues attributed to this group of nematodes as virus vectors.In the present study ultra-stru
为研究生物制剂禾力素对大豆生长及对根部病害的影响，采用包衣、叶面喷施及纳米富硒包衣3种处理方式，测定了不同时期各处理对大豆植株生理活性和大豆孢囊线虫及根腐病防效的影响.结果 表明：生防菌剂禾力素不同处理方式对大豆幼苗生长均有一定促进作用，可以提高大豆幼苗的生理活性，叶绿素、蛋白质、可溶性总糖含量与对照相比，种子包衣处理分别增加了1.53mg/g、0.83mg/g、0.82mg/g；纳米富硒包衣处理
水稻(Oryza sative L.)是我国重要粮食作物之一,植物寄生线虫平均每年导致水稻减产10％～ 25％,并造成严重的经济损失.细尖潜根线虫(Hirschmanniella mucronata)属于潜根属(Hirschmanniella spp.),为水稻重要的内寄生线虫,一般通过除根尖细胞外的根部细胞进入植物体内,在根内皮层和中柱层吸取营养从而影响植株根系的正常发育.本研究利用转录组数据通
短体线虫(Pratylenchus spp.)又称根腐线虫,是一类迁移性植物寄生线虫.短体线虫在植物根系皮层穿刺取食,生活史内可多次侵染,在受害植物根系造成大量伤口,引起根部腐烂,地上部生长衰弱,同时也为其他病原物侵染提供了机会,导致与其他病害复合发生.目前,国内报道的危害农作物的短体线虫主要有咖啡短体(P.coffeae)、落选短体(P.neglectus)、穿刺短体(P.penetrans)等
烟草起源于美洲,在明朝万历年间传入我国.随着烟草吸食量的大幅上升,烟草的种植范围从我国沿海向内地迅速广泛地扩展,逐步形成了黄淮烟区、东北烟区、西南烟区、华中烟区和华南烟区五大产烟区.河南是全国重要的烟草大省,烟草产业基础优势明显,对地方财政和农民增收贡献突出.2012年,河南省卷烟产量338万箱,居全国第二位.2017年,河南省烟叶种植面积达76.24万亩,烟叶主产区烟田多为旱坡地、丘陵地,土质为