【摘 要】
：
A sensitive and specific monoclonal antibody (Mab) against aflatoxin M1 (AFM1) , named as 2C9, was selected by semi-solid HAT medium.It exhibited high affinity for AFM1 of 1.74 × 109 L/mol and no cros
【机 构】
：
Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,China;Key Laborat
【出 处】
：
中国作物学会油料作物专业委员会第七次会员代表大会暨学术年会
论文部分内容阅读
A sensitive and specific monoclonal antibody (Mab) against aflatoxin M1 (AFM1) , named as 2C9, was selected by semi-solid HAT medium.It exhibited high affinity for AFM1 of 1.74 × 109 L/mol and no crossreactivity to aflatoxin B1, B2, G1 and G2.Based on the antibody, an ultra-sensitive competitive enzymelinked immunosorbent assay (ELISA) was developed for AFM1 in milk and infant milk products.Assays were performed in the AFM1-BSA coated (0.062 5 μg/mL) ELISA format in which the antibody was diluted 1∶10000.Several physicochemical factors (pH, ionic strength and blocking solution) that influence assay performance were optimised.Finally, the limits of detection were 3 ng/L for milk and 6 ng/L for milk-based cereal weaning food, inter-assay and intra-assay variations were less than 10%, and the recovery ranged from 91% to 110%.Thirty samples were analysed, and concordant results were obtained when the data were compared with a reference high-performance liquid chromatography method.
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