【摘 要】
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Liquid chromatography and mass spectrometry are enabling technologies for modern proteomics research and therefore,hyphenation of LC-MS has been the primary
【机 构】
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DepartmentofChemistry,XiamenUniversity,Xiamen361005,China
【出 处】
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第八届全国微全分析系统学术会议、第三届全国微纳尺度生物分离分析学术会议暨第五届国际微化学与微系统学术会议
论文部分内容阅读
Liquid chromatography and mass spectrometry are enabling technologies for modern proteomics research and therefore,hyphenation of LC-MS has been the primary workinghorse in practical proteomic analysis.Compared to the relatively mature LC-ESI MS platform,coupling of LC to MALDI MS has been a technical bottleneck for a long while.Droplet technology can convert continuous flow to discontinuous flow,based on which large number of aqueous samples can be separately stored and manipulated.In this project,droplet microfluidics was introduced as a simple and effective approach to connecting capillary liquid chromatography with MALDI MS.With a simple microfluidic structure,HPLC eluent was fragmented into independent fractions with controllable volumes and matrix reagent was introduced at downstream before droplet fractions were loaded onto a MALDI target plate.With proteins as sample,the feasibility of such droplet-interfaced LC-MALDI MS hyphenation was demonstrated and due to the flexible nature of droplets,adjustability of LC-MS resolution was further explored.
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