Tuning spin-related transport properties at the single molecule level is crucial not only for a fundamental understanding of charge and spin interactions but also represents a prerequisite for development of molecular electronics and spintronics.Here we deposited FePc on Au(111)(Figure 1)and then,tuned its spin-related transport properties step by step by single-molecule chemistry.After removing 2H、4H、6H and 8H from the molecule,Kondo effect、spin transition、Kondo effect and spin transition were observed in differential conductance spectra sequentially.