Objective: In order to find the chemotherapy resistance proteins of paclitaxel and carboplatin in cervical SiHa cancer.Methods: We take SiHa cell by protein extraction,quantitative,generation,reductive alkylation,enzyme after 14 days co-culture with paclitaxel and carboplatin.3 standard tags based on the application of iTRAQ reagent LC-MS/MS technology,paclitaxel and carboplatin resistant as study group,Mock group as control group.The quantitative analysis results of Swissprot human library protein quantitative information determined that the differential expression protein is in direct ration greater than 1.2 and p<0.05.Selection the screening biomarkers results of low molecular weight range(1000-50000 Da),within the scope of the molecular weight.We use the lentivirus mediated APOA1 overexpression to infect SiHa cell,qPCR was used to verify the function of paclitaxel and carboplatin chemotherapy resistance.Results: There were total 67 difference proteins by comparing the difference between paclitaxel group and Mock group.At the same time there were total 96 proteins by comparing the difference between carboplatin group and Mock group.GO classification and KEGG 53 proteins(up 43, down 10)(P/M)and 85 proteins(up 70, down 15)(C/M)respectively which have statistically significant differences.We choose APOA1 as target protein of chemotherapy resistance according to literatures,we use CCK-8 to verify overexpression APOA1 can increase paclitaxel and carboplatin chemotherapy-resistance,the IC50 of paclitaxel and carboplatin concentrations were all nearly 2 times higher in overexpression APOA1 SiHa group than in normal SiHa group.Conclusion: iTRAQ technologies based on mass spectrometry based quantitative proteomic methods could able to accurately identify differences proteins of chemotherapy resistance expression in cervical cancer treatment.The APOA1 may be the marker and drug target candidate proteins of chemotherapy resistance.