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This study explores the antimicrobial and enzymatic activities of endophytic fungi isolated from Andrographis paniculata (Hempedu Bumi).This plant is rich with ethnobotanical properties and is an excellent resource for harnessing beneficial endophytes for various applications.In this study, the antimicrobial activities of the endophytes were tested by dual culture screening against five Gram positive, five Gram negative pathogens, the yeast Candida albicans, and two fungal pathogens(Ganoderma boninense, Fusarium oxysporum f.sp.cubense race 4).