Autophagy is an evolutionary conserved lysosome-associated cell process that plays a central role in eukaryotic cell homeostasis.It has been demonstrated that autophagy has not only the ability to degrade intracellular organelles,but also function as an eukaryotic defense mechanism against viral pathogens.On the other hand,viruses have evolved a variety of strategies to evade autophagic attack and even manipulate this machinery for their own survival and propagation within the host.Understanding the interplay between autophagy and viral replication may help us to deep our knowledge of virus-host interaction mechanisms and to potentially develop new targets for viral diseases treatment.To this end,we took HBV and CVB3 as samples to study the relationship between autophagy and viral replication.Our results showed that HBV induced autophagosome formation,which was required for itself replication.