【摘 要】
：
本研究以C57BL/6J ES细胞系为背景,旨在筛选制作模式动物的最佳供体鼠.本实验采用C57BL/6J系ES细胞,以C57BL/6J-Tyrc-2J白化小鼠的囊胚为受体,注射同一种阳性ES细胞,比较其成
【机 构】
：
中国食品药品检定研究院,国家啮齿类实验动物种子中心,北京100050北京百奥赛图基因生物技术有限公司,北京100076
论文部分内容阅读
本研究以C57BL/6J ES细胞系为背景,旨在筛选制作模式动物的最佳供体鼠.本实验采用C57BL/6J系ES细胞,以C57BL/6J-Tyrc-2J白化小鼠的囊胚为受体,注射同一种阳性ES细胞,比较其成功制作模式动物的概率;注射不同阳性ES细胞,比较得到模式动物的概率.结果表明：囊胚注射时,注射B6背景的ES细胞采用C57BL/6J-Tyrc-2J供体鼠囊胚能够得到较高的成功率.因此,当以C57BL/6J ES细胞系为背景,采用C57BL/6J-Tyrc-2J小鼠囊胚,能够提高制作模式动物成功率.
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