【摘 要】
：
大气CO2浓度升高造成海水pCO2升高、pH值降低和碳化学平衡改变,引起海洋酸化.作为钙化生物,贝类的生长和代谢过程可能会受到高CO2浓度条件的影响.酶活性是贝类响应环境变化的重要指标,但其对海水CO2浓度升高的响应的研究鲜有报道.本文采用CO2加富培养实验的方法,研究了魁蚶(Scapharca broughtonii)稚贝3种氧化酶(超氧化物歧化酶SOD、过氧化氢酶CAT和乙酰胆碱酯酶TChE)
【机 构】
：
中国海洋大学 环境科学与工程学院,山东青岛266100 中国海洋大学 环境科学与工程学院,山东青岛
【出 处】
：
2015海峡两岸海洋生物多样性研讨会
论文部分内容阅读
大气CO2浓度升高造成海水pCO2升高、pH值降低和碳化学平衡改变,引起海洋酸化.作为钙化生物,贝类的生长和代谢过程可能会受到高CO2浓度条件的影响.酶活性是贝类响应环境变化的重要指标,但其对海水CO2浓度升高的响应的研究鲜有报道.本文采用CO2加富培养实验的方法,研究了魁蚶(Scapharca broughtonii)稚贝3种氧化酶(超氧化物歧化酶SOD、过氧化氢酶CAT和乙酰胆碱酯酶TChE)活性在高CO2浓度条件下的变化.对照组通入过滤自然空气(395 ppm CO2),实验组通入含1000 ppm CO2的过滤混合空气.培养水体pH和溶解性无机碳(DIC)以及稚贝壳长和酶活性等参数每2天测定一次,培养周期为14天.结果表明,CO2组培养水体中DIC浓度显著高于对照组,魁蚶稚贝初次潜沙时间(5.7 min)相比对照组(7.8 min)缩短,潜沙率(69.7％)相比对照组(61.3％)升高.实验开始时魁蚶稚贝壳长为17.20±0.03 mm,实验结束时CO2组魁蚶稚贝壳未出现明显生长(17.24±0.02 mm),壳长显著低于对照组(17.37±0.07 mm).CO2组魁蚶稚贝SOD活性在培养第2天和第4天时显著高于对照组(p＜0.01),之后出现下降；两实验组魁蚶稚贝CAT活性在前4天均上升,之后CO2组魁蚶稚贝CAT活性基本不变,而对照组出现下降；两实验组魁蚶稚贝TChE活性在培养过程中无明显差异.高CO2浓度条件下魁蚶稚贝壳的生长受到影响,潜沙过程受到促进,三种氧化酶活性在培养过程中的变化趋势存在差异,未来海洋酸化可能会影响魁蚶的养殖.
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