HiSeq高通量测序平台用于白鲜根转录组的数据分析

来源 :陕西省植物学会2018年学术研讨会 | 被引量 : 0次 | 上传用户:jhson47
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
  目的 获得白鲜Dictamnus dasycarpus Turcz.根转录组信息特征.方法 以白鲜根为研究对象,采用Illumina HiSeqTM 2000 150PE 进行高通量转录组测序并进行数据分析.结果 转录组测序共获得69 643 286 条高质量clean reads,Trinity de novo 组装获得49 050个unigenes,平均长度841 nt.BLAST 分析显示分别有31 636(占总unigenes64.49%)、22 367(45.60%)、19 246(39.23%)、12 595(25.68%)个unigenes 在Nr、Swiss-port、KOG、KEGG 数据库得到注释信息,可归为GO 分类的生物过程、细胞组分和分子功能3 大类42 分支,涉及132 个KEGG 标准代谢通路,其中包括18 个次生代谢标准通路.
其他文献
Understanding the processes maintaining tree species diversity is a central problem in ecology,with implications for the conservation and management of forest ecosystems.Although ecologists often assu
Quercitrin,hyperoside,rutin and afzelin are the dominant flavonoids compounds from Zanthoxylumbungeanum leaves and play major roles on the antioxidant activity.Macroporous adsorption resin(MAR)as a si
Water availability is a major constraint for wheat production in many semiarid regions of the world,including the Loess Plateau of China,and thus improving the water use efficiency(WUE)becomes a main
Southwestern(SW)China is an area of active tectonism and erosion,yielding a dynamic,deeply eroded landscape that is hypothesized to have influenced the genetic structure of the resident populations of
In all higher plants studied to date,the phenylpropanoid pathway is regulated by a suit of transcription factors that include MYB,bHLH,WD-repeat proteins,which often forms ternarycomplex.However only
Tapiscia sinensis(Tapisciaceae)has been proved to be a functional androdioecious species with both male and hermaphroditic individuals,and male pollen vitality was far higher than that of hermaphrodit
Forward genetic analysis,widely used to find new gene functions,benefits from the availability of mutants.At present,based on Agrobacterium-mediated plant transformation technology,many transfer(T)-DN
Genome sequencing has demonstrated that besides frequent small-scaleduplications,large-scale duplication events such as whole genome duplication(WGD)are found on many branches of the evolutionary tree
目的 依据42 份国内甘草种质主要的形态性状和化学成分,对其遗传多样性进行综合分析.方法 收集移植于同一地点的多年生甘草种质的12 个地上部分性状和5 种化学成分含量,计算其遗传多样性指数和变异系数,运用聚类分析和主成分分析进行统计分析.结果 5 种化学成分中,甘草苷含量的遗传多样性指数最高为2.05,异甘草苷含量的变异系数最大为99.50%,甘草苷含量与异甘草苷含量呈中度相关关系;在12 个形态
COP1 作为一种E3 连接酶,在暗下其与光形态建成正调节因子HY5 直接互作促使HY5 降解,从而抑制光形态建成,启动暗形态建成。在光下COP1 失活,解除对HY5的抑制,启动光形态建成。在成花诱导的光周期途径中,前人研究表明COP1 通过与CONSTANS(CO)直接互作促使其降解,进而抑制开花整合子FLOWERING LOCUS(FT)、TWIN SISTER OF FT(TSF)和SUPP