Aims:Based on the self-assembled properties of amphiphilic polymers and HA-mediated tumor targeting,amphiphilic hyaluronic acid (HA)-based derivative was developed as a carrier of paclitaxel (PTX) to improve the solubility of PTX and facilitate its tumor targeting delivery.Methods:PTX-loaded polymeric nanoparticles were prepared by the dialysis method.The effect of degree of substitution (DS) of hydrophobic section in polymers on drug loading,entrapment efficiency and particles size were investigated.The in vivo drug release was also evaluated.In addition,confocal microscopy was used to internalize the intracellular location ofnanoparticles.