The BiobricksTM standard has rendered the construction of DNA modules easier,quicker and cheaper.So far, over 100 BioBricksTM assembly schemes have been developed and many of them, including the original standard of BBF RFC 10, are now widely used.However, since the restriction endonucleases employed by these standards ususlly recognize short DNE sequences that are widely spread among natural DNA sequences, and thus must be removed before the parts construction,there is much inconvenience in dealing with lsrge-size DNA parts with the present standards.Here, we introduce a new standard, named iBrick, which uses two homing endonucleaes of I-SceI and PI-PspI.As both enzymes recognize long DNA sequences (>18 bps), their sites are extremely rare in natural DNA sources, thus providing additional convenience, especially when handling large pieces of DNA fragments.Using the iBrick standard, the carotenoid biosynthetic cluster (>4 kb) was successfully assembled and the actinorhodin biosynthetic cluster (>20 kb) was easily cloned and heterologously expressed.In additon, a corresponding nomenclature system has been extablished for the iBrick standard.