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  5. MicroRNA-21/PTEN/AKT介导曲美他嗪在缺氧/复氧诱导H9C2细胞凋亡中的抗凋亡作用

MicroRNA-21/PTEN/AKT介导曲美他嗪在缺氧/复氧诱导H9C2细胞凋亡中的抗凋亡作用

来源 :第二十四届长城国际心脏病学会议、亚太心脏大会暨国际心血管病预防与康复会议(The 24th Great Wall Int | 被引量 : 0次 | 上传用户：chijr
【摘 要】
：
目的:探讨曲美他嗪在大鼠心肌缺血再灌注损伤早期的抗细胞凋亡作用是否通过microRNA-21(miR-21)/PTEN/AKT途径介导.方法:H9C2细胞随机分为对照组、缺氧4h复氧3h组、缺氧4h复氧3h+曲美他嗪组,用荧光定量PCR检测各组miR-21表达变化;流式细胞术检测各组细胞凋亡率,以Bax/Bcl-2、caspase 3作为凋亡相关指标,Western印迹方法检测Bax/Bcl-2、
【作 者】
：
杨琼 杨侃 
【机 构】
：
中南大学湘雅三医院
【出 处】
：
第二十四届长城国际心脏病学会议、亚太心脏大会暨国际心血管病预防与康复会议(The 24th Great Wall Int
【发表日期】
：
2013年2期
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目的:探讨曲美他嗪在大鼠心肌缺血再灌注损伤早期的抗细胞凋亡作用是否通过microRNA-21(miR-21)/PTEN/AKT途径介导.方法:H9C2细胞随机分为对照组、缺氧4h复氧3h组、缺氧4h复氧3h+曲美他嗪组,用荧光定量PCR检测各组miR-21表达变化;流式细胞术检测各组细胞凋亡率,以Bax/Bcl-2、caspase 3作为凋亡相关指标,Western印迹方法检测Bax/Bcl-2、caspase-3水平;PTEN是已证实的miR-21靶基因,Western印迹方法检测miR-21下游通路PTEN/AKT蛋白水平.进一步用miR-21inhibitors抑制miR-21表达后,观察对缺氧4h复氧3h组+曲美他嗪组细胞凋亡水平及下游通路PTEN/AKT水平的影响.
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