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CrylFa-expressing corn and cotton have been commercially planted in U.S in order to effectively control several Lepidoptera pests.Making clear the cross-resistance and synergism between Cry1Ac and Cry1Fa could provide the theoretical bases for rationally applying Cry1Fa+Cry1Ac cotton.The toxicity of Cry1Fa to the susceptible (96S) and resistant (BtR, selected by Cry1Ac, the resistance ratio was 2 194.15-fold) cotton bollworms (Helicoverpa armigera) was tested in our study.