Euchiloglanis kishinouyei is a demersal freshwater fish belonging to the family Sisoridae in Siluriformes.It was originally described from the upper Yangtze drainage in China.In recent years,the species has been in rapid decline because of overfishing and the loss of habitat following the completion of several dams along the upper Yangtze River and its tributaries.However,the available genetic data for this species is limited.In the present study,sixteen novel polymorphic microsatellite loci were isolated and characterized for Euchiloglanis kishinouyei and tested in a natural population with 40 individuals.The number of alleles ranged from 3 to 17,the observed and expected heterozygosities ranged from 0.030 to 0.950 and from 0.163 to 0.980,respectively,and the average polymorphic information content (PIC) of all loci was 0.450.In addition,there was a high level of cross-amplification at 63 loci against three other sisorid catfishes: Pareuchiloglanis kamengensis,Pareuchiloglanis sinensis,and Glyptothorax fokiensis,which suggests that the microsatellite markers developed for E.kishinouyei could also be used for related sisorid catfish species.