Cells in vivo are continuously and simultaneously exposed to the different types of stimulations,which,in turn,trigger cellular responses important to the cells biologicalfunctions.Biochemical,mechanical,electrical,surface (topological or surface matrixrelated),cellular (cell-to cell interactions,spheroids),and physical (temperature,magnetic,optical) stimuli are the representative,and it is a great challenge to mimic these envi-ronment under in vitro conditions.Inthis presentation,we present several microfluidic platforms to control microenvironment including chemical gradient,flow speed compa-rable to interstitial flow and curved substrate.Theslowly flowing chemical gradient m-imics the interstitial flow in the body.In most of gradient chips,achievement of such slow flow is rare,but,we have developed the gradient chips with slow flow using osmotic pumping system.With this platform,we have observed the embryo stem celldifferentiation to neuron cells,culture of human mesenchymal stem cells (hMSC) to the amount of nutrient,and hMSC differentiation to bone cells.We have also develo-ped the gradient chip to the 3D tissue model.In this chip,the 3D engineered sphero-id tissues were cultured,and the signaling molecules were delivered to the 3D tissues.This model can be used toinvestigate the pathways of specificsignaling molecules and cell-cell touch effect.In addition,the flow effect can be observed.The proposed microfluidic platform can be extensively used for the drug or molecule discovery withmore in vivo mimicking 3D engineered tissue model.In addition,the study for cellul-ar behavior under the specific environments can be achieved easily with simple platf-orm.