Fulminant hepatic failure (FHF), a liver disorder that often leads to devastating consequences, is one of the most challenging gastrointestinal emergencies encountered in clinical practice, and encompasses a pattern of clinical symptoms and pathophysiological responses associated with the rapid deterioration of normal hepatic function.In this study, gas chromatography-orthogonal acceleration time of flight mass spectrometry (GC-oaTOF MS) were used to determine the compounds in mouse plasma to characterize the changes in metabolic profiles of a mouse model with FHF, induced by D-galactosamine/lipopolysaccharide (D-Gal/LPS).