Objective Local inject different concentrations of hydrochloric acid(HCl)in rabbit live and studywhether it would effect rabbits general situation,眀愀琀攀爀,electrolyte and acid-base balance,routine blood,liver and kidney function,adjacent organs through hematology test,imaging and histopathological study.Further discuss whether HCl is a safety local injection chemical ablation agent昀爀漀洀 general and local security.Materials and Methods Eighty adult New Zealand rabbits were divided into five groups of equal size(n=16),i.e.Group A: untreated group,Group B:normal saline(NS)control group,Group C: 10%HCl group,Group D: 20%HCl group,Group E:30%HCl group.Except group A injected none liquid in the liver,other groups injected 0.5ml of NS,10%HCl,20%HCl,30%HCl in the liver,respectively.Each group was divided into two subgroups: ten rabbits for toxicology study and and six rabbits for imaging and histopathology study.In toxicology study we collected 2ml blood sample at the time points of hour 0,0.25,0.5,1,3,6 and day 2,7,14.Among these 2ml blood sample,0.5ml for愀爀琀攀爀椀愀氀 blood gas,0.5ml for hematologyand1ml for hepatic and renal functions.General condition was recorded at the time points of day 0,2,5,7,14.In imaging and histopathology study,Each rabbits undergo MRI scan at the time points of day 2,7 and 14.After each MRI scan,each group execute two rabbits to get liver specimen.We observed the the sizes of lesion and the histopathology change that caused by HCl.Results The food consumption,water consumption and body weight decreased significantly in all group at day 2(P< 0.0001),but all of them recovered at day 7.In blood gas analysis,the lactic acid content was significant higher in the group E than in the other groups while the actual base excess眀愀猀 significant lower in the group C(P<0.0001).K+,一愀+,Ca2+,PH,Lac,HCO3,actual base excess栀愀搀 no significant differences in interaction between group effect and time effect and had no猀椀最渀椀昀椀挀愀渀琀 differences in group effect.In blood routine,WBC,RBC,PLT,HGBwere no significant differences in interaction between group effect and time effect and had no猀椀最渀椀昀椀挀愀渀琀 differences in group effect.In liver and renal function,ALT and AST were significant higher in group E than in other groups(P<0.0001).吀倀,ALB,A/G,CRP,BUN,CRE had no significant differences in interaction between group effect and time effect and had no猀椀最渀椀昀椀挀愀渀琀 differences in group effect.In time effect,all items expect A/G had significant change during experiment.However these changes were reversible and recoverable at day 14.In MRI,groupA and B,no obvious lesions were observed on T2WI at all three time points.In group C,D and E,the lesions were homogenously hyperintense on T2WI.Inpathology study,group A and B had no猀椀最渀椀昀椀挀愀渀琀 pathological changes.In group C,D and Ethe cellular structure was gradully氀漀猀琀眀椀琀栀 time past.On搀愀礀14,琀he cellular structure was挀漀洀瀀氀攀 琀攀氀礀氀漀猀琀愀渀搀 only a small amount of the residual nuclear structure was observed at a high magnification.At day 2,coagulated necrosis was observed in the area of the local injection with HCl in the liver,and no evident adhesion to the adjacent organs was observed.At days 7 and 14,the damaged area was surrounded by a layer of fiber tissue.The damaged area seriously adhered to the surrounding tissue and causing the separation of them became very difficult.Conclusions 匀氀漀眀 injection(no more than 1 ml/min)HCl that concentration was under 30%and no more than 0.5 ml in the rabbit liver was safe.This process will not affect the water – electrolyte and acid-base balance,blood routine,renal functions.But it will cause the reversibility of liver function damage.HCl homogeneous diffuses in the rabbits liver and it will generate minor damage area that aroud completely coagulated necrosis.吀栀攀 minor damaged area in the 30%HCl group was larger than in the 10%、20%HCl group.