Background Acute acalculous cholecystitis (AC) is absence of specific clinical manifestations and difficult to be diagnosed.It is a kind of serious illness, onset acute and progress fast.The mortality rate of AAC remains at least 30％.The pathogenesis of AC is not completely elucidated.Impaired smooth muscle contractility is a hallmark of AAC.Interstitial cells of Cajal (ICC) in the gastrointestinal (GI) tract is considered to be pacemaker cells for slow wave and regulates the rhythmic smooth muscle contraction in the GI tract.Rhythmic Ca2+ transient in ICC is crucial in pace-making.