Peanuts are an increasingly important global food source.However,until recently the lack of effective protocols for the extraction of nucleic acids has made molecular studies of peanut development and maturation difficult.Here we describe a method to isolate high-quality RNA and DNA from peanut tissue and have successfully applied this method to peanut plant roots,stems,leaves,flowers,and seeds.Spectrophotometric analysis showed that the average yields of total RNA from 100 mg of peanut materials ranged from 24.52 μg to 74.6 μg,and those ofgenomic DNA from the same tissues ranged from 23.47 μg to 57.68 μg.