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Breastmilk has an effective function on preventing infection.β-casein65 is a novel endogenous peptides found in preterm milk by Liquid chromatography-mass spectrometry/mass spectrometry technique.Our experiments confirm that β-casein65 has strong antibacterial activity on common newborn infection bacteria such as S.aureus and E.coli by agarose filter paper diffusion method and microdilution method.We use Live/Dead Baclight Bacterial Viability Kit test S.aureus and E.coli treated with β-casein65.