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Recycling high speed counter-current chromatography (HSCCC) was successfully applied to resolution of2-phenylpropionic acid enantiomer using hydroxypropyl-β-cyclodextrin (HP-β-CD) as chiral selector.The two-phasesolvent system composed of n-hexane-ethyl acetate-0.1 mol-1 phosphate buffer solution with pH=2.67 (8:2:10,v/v/v)was selected.Influence factors involved in the chiral separation were investigated,including the concentration ofHP-β-CD,pH value of the aqueous phase,the separation temperature.Suitable elution mode was selected forHSCCC enantioseparation of (±)-2-phenylpropionic acid.Under optimum separation conditions,50 mg of (±)-2-phenylpropionic acid was separated using preparative recycling HSCCC with multiple recycling elution mode.Thepurities of both of the fractions including (+)-2-phenylpropionic acid and (-)-2-phenylpropionic acid from thepreparative CCC separation were over 92.5% determined by HPLC.