Quantitative trait locus (QTL) analysis of natural Arabidopsis accessions in sugar sensitivity was undertaken in this study.A previously unidentified glucose sensing QTL, GSQ11, was mapped on chromosome 3 through selective genotyping in CoL/C24 F2 population and confirmed in near-isogentic lines in both Col and C24 background.Among the causal genes in the candidate region the T-DNA insertion mutant of the Arabidopsis NAC transcription factor 060 (ANAC060) showed glucose supersensitive phenotype.