Human mesenchymal stem cells (hMSCs) hold great promise in cell therapy and regenerative medicine.Various preclinical and clinical trials have been carried out to illustrate the therapeutic potential of these cells.However, one major challenge for manufacturing clinical grade hMSCs is the requisition of current good manufacturing practice (cGMP) grade practices in cell isolation, processing, storage, and distribution.Development of non-toxic and animal serum-free preservation medium is critical for storage and distribution of mesenchymal stem cells (MSCs).In this study, we developed a solution formula that could preserve MSCs at 4℃ for up to 3 weeks.In the solution, trehalose is a key ingredient for maintaining survival of MSCs.Among the concentrations investigated, 40 mM trehalose showed the best outcome with the viability maintained more than 92.7±1.5% for 7 days.Cells preserved in the solution formula for 3 weeks still remained about 70% viability, and produced results similar to those of freshly harvested hMSCs in terms of growth kinetics, expression profile of cell surface antigens, and differentiation potential.In summary, storage of MSCs in the medium makes it far easier for transporting the cells from processing units to clinical sites.