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目的:评价PEB1介导的屋尘螨抗原(Derp2)重组BCG疫苗(PEB1-Derp2-rBCG)与人上皮细胞的结合能力。方法:采用体外细胞培养的方法,分别将普通BCG、胞壁型Derp2-rBCG和胞壁型融合蛋白PEB1-Derp2-rBCG与HeLa细胞及人类肠粘膜上皮细胞(HIEC)进行共孵育,利用HE和抗酸染色法对各组细胞与疫苗的黏附结果进行染色,光学显微镜下计数各组的黏附率,并进行比较;对以上各组分别加入PEB1蛋白,进行黏附阻断,观察对结合能力的影响。结果:孵育24小时后,无论HeLa细胞还是HIECPEB1-Derp2-rBCG组较普通BCG组和Derp2-rBCG组的黏附率明显提高,差异有显著性(P<0.05);PEB1蛋白的加入对PEB1-Derp2-rBCG的黏附功能有明显抑制作用(P<0.05);但是,Derp2-rBCG组与普通BCG组比较没有明显差异(P>0.05),PEB1蛋白的加入对二者的黏附亦无影响(P>0.05)。结论:PEB1具有介导增强PEB1-Derp2-rBCG与上皮细胞黏附的能力。
PURPOSE: To evaluate the binding of PEB1-mediated binding of Derp2 recombinant BCG vaccine (PEB1-Derp2-rBCG) to human epithelial cells. METHODS: Common BCG, Derp2-rBCG and PEB1-Derp2-rBCG were co-incubated with HeLa cells and human intestinal epithelial cells (HIEC) respectively in vitro. HE and The adhesion results of cells and vaccine in each group were stained by acid-fast staining. The adhesion rate of each group was counted under light microscope. PEB1 protein was added to the above groups to block the adhesion and observe the effect on the binding ability . Results: The adhesion rate of HeLa cells or HIECPEB1-Derp2-rBCG group was significantly higher than that of normal BCG group and Derp2-rBCG group after 24 hours of incubation (P <0.05); PEB1-Derp2 (P <0.05). However, there was no significant difference between the Derp2-rBCG group and the normal BCG group (P> 0.05). The addition of PEB1 protein had no effect on the adhesion (P> 0.05). Conclusion: PEB1 has the ability to mediate the adhesion of PEB1-Derp2-rBCG to epithelial cells.