The lateral habenula (LHb) is considered as a negative reward regulator and it become active when an organism fails to receive an expected reward or if the organism receives a signal that indicates a negative outcome.Neurons in LHb become active when an organism fails to receive an expected reward or the organism receives a signal indicating a negative result.However, the mechanism of potential modulation of LHb neurons in a negative reward regulation are poorly understood.In this study we assessed electrophysiological responsiveness of LHb neurons on stimulation of relative structures in central nervous system in the Wistar rats.Stimulation of the specific relative structures in central nervous system elevated or inhibit firing rate in most of the LHb neurons, which conform neural circuits.So, LHb take part in neural circuits as a negative regulator.