Wntβ-catenin信号通路诱导人表皮干细胞向成釉质细胞分化

来源 :中国细胞生物学学会2015年全国学术大会 | 被引量 : 0次 | 上传用户：yanzhijianer

【摘要】

：

　　人表皮干细胞(human keratinocyte stem cells, hKSCs)可作为上皮源性的成体干细胞应用于牙齿再生,但是其诱导效率较低.本研究利用小分子化合物CHIR-99021以及过表达β-c

【作者】

：

刘英林程琳林鑫王冰梅

【机构】

：

福建师范大学生命科学学院,福建省发育与神经生物学重点实验室,福州,350108

【出处】

：

中国细胞生物学学会2015年全国学术大会

【发表日期】

：

2015年4期

【关键词】

：

人表皮干细胞牙齿再生 Wnt/β-catenin信号通路 CHIR-99021 β-catenin

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论文部分内容阅读

　　人表皮干细胞(human keratinocyte stem cells, hKSCs)可作为上皮源性的成体干细胞应用于牙齿再生,但是其诱导效率较低.本研究利用小分子化合物CHIR-99021以及过表达β-catenin基因在人表皮干细胞的表达,提高hKSCs的Wnt/β-catenin信号活性,再与具有诱导成牙潜能的小鼠牙胚间充质重组,构建嵌合体并移植裸鼠肾囊膜下培养20 d.将嵌合体组织切片,并利用组织染色和免疫组化等方法鉴定牙齿结构.

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Wntβ-catenin信号通路诱导人表皮干细胞向成釉质细胞分化

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