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Metastasis remains the primary cause of prostate cancer related death.Here,we performed gene expression profiling of Ephs and their ephrin ligands in well-established prostate cancer cell lines LNCaP and PC-3 and their derivative lymph node metastasis sub-cell lines.Among the tested Eph receptors and ligands,EphA6 is the only consistently overexpressed member in lymph node metastatic cell lines.