Engineering of the surface is the multidisciplinary research of materials science and has a broad range of applications to chemistry,biology,engineering and medicine.Surface modification of a biomaterial can be done by different methods for altering into required characteristics,such as size,morphology,topology,wettability,roughness,surface charge,reactivity,biocompatibility and applicability.Tissue engineering is the use of a combination of cells,engineering and materials methods,and suitable biochemical and physicochemical factors to improve or replace biological functions of tissues.Here we provide an overview of the various applications of our surface modification systems and also discuss successes to date,current limitations and future directions.We developed surface modification system of nanotubes,nanoparticles,polymers,plastic wares,polydimethylsiloxane chips,using chemicals,zwitterionic polymers,polyethylene glycol(PEG),3-aminopropyltriethoxysilane(APTES),extracellular matrix(ECM),other bioactive molecules,etc for efficiently enhancing cell functions.Our results highlight an innovative surface-modified biomaterials for advance cell culture and tissue regeneration system and may allow the development of enabling technologies for tissue engineering and regenerative medicine applications.