Establishment of direct detection method for serum circulating Bmi-1 mRNA and its application in dia

来源 :中华医学会第八次全国中青年检验医学学术会议 | 被引量 : 0次 | 上传用户：liuzufang

【摘要】

：

Objective To establish a direct reverse transcription real-time fluorescence quantitative polymerase chain reaction (RT-qPCR-D)method for detecting serum circulating Bmi-1 mRNA,and analyze the levels

【作者】

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Xin Zhang Chuanxin Wang

【机构】

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Department of Clinical Laboratory,Qilu Hospital of Shandong University,Jinan,250012,China

【出处】

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中华医学会第八次全国中青年检验医学学术会议

【发表日期】

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2014年6期

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Objective To establish a direct reverse transcription real-time fluorescence quantitative polymerase chain reaction (RT-qPCR-D)method for detecting serum circulating Bmi-1 mRNA,and analyze the levels of serum circulating Bmi-1 mRNA in colorectal cancer patients using this method for exploring its diagnosis value in colorectal cancer.Method RNA was extracted from colorectal cancer HT29 cell line,and detection standard curves of Bmi-1,GAPDH,EEF1A1 mRNAs were established,then the amplification efficiencies were calculated.

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