Enantiomerically pure sulfoxides play an important role in asymmetric synthesis either as chiral building blocks or as stereo directing groups.Asymmetric oxidation of a prochiral sulfide is the most direct and economical method for the synthesis of enantiomerically pure sulfoxides[1] In our previous work, we have isolated a Gram-positive bacterium, Rhodococcus sp.ECU0066, displaying a novel sulfide oxidation activity[2].