A simple, reliable method for simultaneous determination of the ten components in Dannong injection (DHI) in combination of chromatographic fingerprint analysis was developed by high performance liquid chromatography coupled with diode-array-detector (HPLC-DAD).The separation was performed on an Agilent Zorbax SB-C18 column with a linear gradient elution of acetonitrile and 0.5% formic acid.The method was validated by linearity, precision, stability and recovery.The developed method was subsequently applied to evaluate 15 batches of DHI and testified to be suitable for its quality control.