【摘 要】
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Objective To investigate the effects of silica nanoparticles in expression profiles of membrane proteins in HaCaT cells.Methods We utilized a subcellular fr
【机 构】
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Key Laboratory of Modern Toxicology of Shenzhen, Medical Key Laboratory of Guangdong Province, Medic
【出 处】
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中国毒理学会生化与分子毒理专业委员会学术会议暨环境内分泌干扰物的毒理学研究新技术新方法研讨会
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Objective To investigate the effects of silica nanoparticles in expression profiles of membrane proteins in HaCaT cells.Methods We utilized a subcellular fractionation method to extract membrane fractions and analyzed the membrane proteomic via a two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization tandem time-of-flight in the membrane fractions of HaCaT cells treated with silica nanoparticles.Results Ten proteins were identified as differential expression in the membrane fractions of HaCaT cells treated with silica nanoparticles.Among these, 7 proteins were predicted as membrane proteins with at least one transmembrane domain.The most dominant function of the identified proteins was binding and the dominant protein class is cytoskeleton proteins.Conclusion Silica nanoparticles exposure can induce the differential expression of membrane-associated proteins.We anticipate that our findings can provide novel scientific clues to discover membrane biomarkers and elucidate membrane proteins-associated toxicological mechanisms of silica nanoparticles.
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