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AIM To observe the inhibition of the extract of Radix scutellariae and Trollius chinensis on four kinds of influenza viruses in vitro.METHODS Cytopathic effect (CPE) observation was used to observe the inhibitory effect of the extract of Radix scutellariae and Trollius chinensis on influenza a (H1N1) virus FM1 strain, influenza a (H1N1) virus PR8 strain, influenza a (H3N2) virus brisbanel0 strain and influenza B virus Xiushui strain.RESULTS The extract of Radix scutellariae and Trollius chinensis had inhibiting effects on cytopathic effect of MDCK cells caused by the infection of H1N1 virus PR8 strain, influenza a (H3N2) virus brisbanel0 strain and influenza B virus Xiushui strain, and their 50% inhibiting concentration (IC50) were 0.67, 0.47and 0.67 g· L-1.CONCLUSION The extract of Radix scutellariae and Trollius chinensis has antiviral effect on influenza viruses in vitro.