The purpose of this experiment was to study the effect of BCB staining on IVM-1VF of cow oocytes. The immature oocyte was stained by BCB, then they were collected by activity of 6-glucose phosphate dehydrogenase. Cumulus oocyte complexes (COCs) were obtained from slaughterhouse-collected bovine ovaries and divided into two groups: Control group which were cultured without exposure to BCB stain; unmatured oocyte of treatment group were stained by BCB for 90min, because BCB staining caused Metabolism of 6-glucose phosphate dehydrogenase, with the observation of stereoscopic microscope, blue group and achromatic color group was as experiment group according to cytoplasmic color. Concentration of glutathione was measured before and after maturation. At last, oocyte was fertilized in vitro after cultured to maturation, cleavage rate after insemination for 48h and blastula development rate after cultured 7-8d were studied. The results showed that (1) maturing rate of blue color group (80.9％) was higher than control group (60.2％) and cytoplasmic color group (51.9％); (2) Concentration of glutathione of mature oocyte had significant difference from oocytes before culture, and Concentration of glutathione of oocyte before and after culture (5.04; 10.98pmol/cell) had significant difference from control group (3.80; 7.42 pmol/celland) colorless group (3.51; 7.29pmol/cell), Concentration of glutathione of mature oocyte was 2 times of nearly mature oocyte; (3) blastula development rate of matured oocyte in blue color group (32.99％) had significant difference from control group (17.64％) and colorless color group (3.57％). BCB staining could be one of the criterion of selecting good oocyte; In the oocyte, Concentration of glutathione was a key Biological indicators of evaluating mature degree of cytoplasm and nucleolus in mammalian.