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以鹿角盘为原料,经过酶水解提纯鹿角盘蛋白多肽(CPP),并对影响酶水解过程的各个因素进行分析。通过Folin酚法和水合茚三酮法鉴定鹿角盘多肽。SDS-聚丙烯酰胺凝胶电泳法测定鹿角盘蛋白多肽的分子质量分布在8～15kD之间。活性试验结果表明,鹿角盘蛋白多肽具有明显增加小鼠吞噬功能及抗大鼠乳腺增生的作用。
The antler plate was used as the raw material to purify the anthelmintic plate protein (CPP) by enzymatic hydrolysis. The factors affecting the enzymatic hydrolysis process were analyzed. The antler plate polypeptide was identified by Folin’s phenol method and ninhydrin method. SDS-polyacrylamide gel electrophoresis determination of the antler plate protein polypeptide molecular weight distribution between 8 ~ 15kD. Activity test results show that the antlers protein can significantly increase phagocytosis in mice and anti-rat mammary gland hyperplasia.