The Efficacy of Tissue Engineering Skin in Applying The Healing of Chroinic Ulcers Kun Song1,2,Jie Luo1,2,Lu Yang1,2,Yongjie Zhang1 Extensive defect of skin is one of common and intractable diseases in clinic,people desired to find ways to resolve the problem from early ago.Patients with chronic skin ulcer caused skin defect,surrounding tissue and cell dysfunction,insensitive to conventional therapies,due to systemic or local factors such as excessive blood sugar,ischemia of extremity end,nervous lesion,abnormal immunity and infection.During clinical observation patients with of wound infection,gangrene,long course,even amputation will suffer unacceptable pain.This good clinic application method of two layers tissue engineered active skin(ActivSkin)on the great scale diabetic ulcer are discussed in the paper.The two layers tissue engineering active skins were prepared using the epidermal cells and fibroblasts from the same race as epidermal seeds cells and dermal seeds cells.The chronic ulcer wound could be repaired by tissue engineering skin in prompting the early healing,shortening the ill course and reducing its complications.It is practical and feasible for clinical application of tissue engineering skin.