Melon,Cucumis melo L.is an important vegetable crop worldwide.At present,there are phenomena of homonyms and synonyms in the melon seed market of China,which could cause variety authenticity issues influencing the process of melon breeding,production,marketing and other aspects.Molecular markers,especially microsatellites or simple sequence repeats (SSRs) are playing increasingly important roles for cultivar identification.The aims of this study were to construct a DNA fingerprinting database of major melon cultivars,which could provide a possibility of establishment of a technical standard system for purity and authenticity identification of melon seeds.In this study,to develop the core set SSR markers,470 polymorphic SSRs were selected as the candidate markers from 1219 SSRs using 20 representative melon varieties (lines).18 SSR markers evenly distributed across the genome and with higher contents of polymorphism information (PIC) were identified as the core marker set for melon DNA fingerprinting analysis.Fingerprint codes for 471 melon varieties (lines) were established.There were 17 groups involving 51 materials with same fingerprint code,while field traits survey results showed that these materials were synonyms.Furthermore,DNA fingerprinting quick response (QR) codes of 471 melon varieties (lines) were constructed.Due to its fast readability and large storage capacity,QR coding melon DNA fingerprinting is in favor of read convenience and commercial applications.