Chlorotoluron and prometryne are the pesticides that are moderately absorbed and fairly mobile and leaching in soils. Dissolved organic matter（DOM) plays a critical role in affecting the environmental behavior of pesticides. In this study，we report the effect of DOM extracted from sludge（SL) and straw（ST) on chlorotoluron and prometryne sorption/desorption and mobility using several techniques including batch experiment，soil column，soil thin-layer chromatography and bioefficacy estimation. Application of DOMs（SL and ST) decreased the sorption of and increased desorption of chlorotoluron and prometryne in soils compared with the control.