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A monolithic molecularly imprinted polymer (monofithic MIP) for dibenzoyl-D-tartaric acid (D-DBTA) was prepared by in situ polymerization as HPLC stationary phase.By optimizing polymeric and chromatographic conditions,the chiral separation of DBTA enantiomers was successfully achieved on this kind of MIP in less than 25 min with a resolution Rs=1.25,whereas no enantioseparation effect was found on the monolithic non-imprinted polymer (NIP).