【摘 要】
：
A new and simple high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) method for determination of Kryptofix 2.2.2 (K-222) in the radiopharmaceuticals of 2-deoxy-2-[
【机 构】
：
Department of Pharmacy,Guangzhou General Hospital of Guangzhou Military Command,Guangzhou,510010,PR
论文部分内容阅读
A new and simple high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) method for determination of Kryptofix 2.2.2 (K-222) in the radiopharmaceuticals of 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) was developed.A C18 column was used and the mobile phase was methanol:0.1%TFA (10∶ 90) at a flow rate of 0.2ml/min.The drift tube temperature was 40 ℃.The pressure of nebulizing gas (N2) was 3.0 bar.The gain was 10.Good separation of K-222 from main related substances could be achieved.Excellent linearity (R2 =0.9995) was obtained over the range of 5-100 μg/ml.The precision ranged from 0.68% to 5.16% (RSD) and the accuracy ranged from 98.20% to 105.16% (RE).The limit of detection was 2 μg/ml.This method offers simple,rapid,quantitative and sensitive detection of K-222 in [18F]FDG thus making it amenable to routine quality assurance testing.
其他文献
The situation of career identity and job satisfaction among young hospital pharmacists in Guangdong,China was investigated.A mail-based questionnaire was sent to young hospital pharmacists in Guangdon
本文的目的是利用大鼠研究糖尿病皮肤对脂溶性的醋酸地塞米松(DA)和水溶性的地塞米松磷酸钠(DSP)经皮渗透和吸收作用的影响.在研究中采用了病理学方法观察糖尿病皮肤的病理改变,还同时引进了改良型Franz扩散池及微透析来评价上述两种药物的经皮渗透及吸收,并采用了高效液相或者液质联用的方法测定样品中DA和DSP的含量.结果表明,与正常大鼠相比,DA经糖尿病皮肤的渗透和吸收量无明显变化,而DSP经糖尿病
目的：比较三七总皂苷(PNS)与比索洛尔对急性心肌梗塞(AMI)后左室重构(LVRM)大鼠心功能作用.方法：除假手术组外采用结扎大鼠左冠状动脉前降支的方法建立AMI模型,术后24h后随机分为对照组和实验组,连续4w分别灌胃给予生理盐水2mg.kg-1、比索洛尔25mg.kg-1和PNS40、80、120 mg·kg-1,观察PNS和比索洛尔对病鼠室间隔舒张末期厚度(IVSd)、左室舒张末期内径(宽
目的:探讨哈蟆油(oR)对D-半乳糖所致雌性衰老大鼠肝脏组织p16、p21和cyclinD1蛋白表达的影响,进一步探讨OR延缓雌性大鼠机体衰老机制.方法：SPF级SD雌性青年大鼠40只随机分为模型组(D-gal组)、维生素E(VE组)、哈蟆油高剂量组(OR-H组)、中剂量组(OR-M组)、低剂量组(OR-L组),每组8只,D-半乳糖颈背部皮下注42d,建立亚急性衰老模型.另取雌性青年大鼠8只,同样
目的：研究芹菜素与柚皮素分别经大鼠空肠、回肠与结肠黏膜的体外区段透过特征.方法：采用体外垂直扩散池(Ussing Chamber)方法,分别评价50 μmol·L-1芹菜素及柚皮素经空肠、回肠和结肠黏膜经时(O～120min)吸收方向(M-S)与分泌方向(S-M)的累计透过率,并通过体系稳态时间(60min-120min)的累计透过率计算各区段吸收方向与分泌方向的表观渗透系数(Papp).芹菜素与
Purpose:The aim of this study was to test the ability of paclitaxel-loaded poly(butyl) cyanoacrylate (PBCA) nanoparticles to overcome multidrug resistance (MDR) in human ovarian resistant cells (A2780
Bone marrow mesenchyrnal stem cells (BMSC) can differentiate into diverse cell types,including adipogenic,osteogenic,chondrogenic and myogenic lineages.There are lots of BMSC accumulated in atheroscle
目的:探讨复方姜黄合剂对糖尿病大鼠视网膜新生血管的干预效果,并研究其剂量一疗效的关系.方法:STZ(链脲佐菌素)诱导糖尿病大鼠成功后,实验鼠随机分为合剂低剂量组、合剂中剂量组、合剂高剂量组、模型组、羟苯磺酸钙组,并设正常对照组.合剂组和羟苯磺酸钙组分别用复方姜黄合剂和羟苯磺酸钙,模型组和对照组用等容量蒸馏水灌胃.20周后,将实验大鼠处死.取眼球行视网膜消化铺片,观察血管形态,微血管数目,计算每高倍
Backbround:Venlafaxine is a new antidepressant that has a chemical structure and neuropharmacologic profile distinct from those of existing antidespressants.The studies about the mechanism of pharmaco
The amyloid precursor protein (APP) is cleaved enzymatically by nonamyloidogenic and amyloidogenic pathways.α-Secretase cleaves APP within β amyloid protein (Aβ) sequence,resulting in the release of a