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Background, Motivation and Objective Chordal cutting is a technique that has been developed recently to improve the efficacy of ischemic mitral regurgitation (MR) surgery. The technique relies on the surgical section of one type of mitral chordae and is achieved during open chest procedure with the heart stopped and extracorporeal circulation (ECC). In this study we investigated the feasibility of using histotripsy for non-invasive chordal cutting on ex vivo chordae samples and in vivo beating hearts in a model of large animal.