Background, Motivation and Objective Gas vesicles (GVs) are gas-filled nanostructures generated by species of archaea and bacteria as a means to regulate buoyancy. A preliminary study showed that GVs embedded in an Agar matrix exhibit a non linear behavior when exposed to ultrasound in the 12.5-27.5 MHz frequency range, and a collapse pressure around 500 kPa. This collapse pressure is more than twice higher than the collapse pressure of Halo GVs in solution in hydrostatic conditions reported in the literature (50-200 kPa). The present study investigates the influence of the surrounding media on GVs collapse pressure and acoustic behavior.