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Objective:To identification Angellica sinensis (Oliv) Diels (Chinese name:Dang-gui).and four medicinal plants,Angelica acutiloba (Sieb.et Zucc.) Kitagawa(Chinese name:Dong-dang-gui),Angelica pubescens Maxim.f.biserrata Shan et Yuan (Chinese name:Du-huo),Levisticum officinale Koch(Chinese name:Ou-dang-gui),Ligusticum Chuanxiong Hort.(Chinese name:Chuan-xiong),which are easily confused when used cure disease in China.Methods:The characteristic markers at the proteomic,phylogenetic,and genomic structural levels for Angellica sinensis (Oliv) Diels.and the four different herb samples were identified by four different methods,sodium dodecyl sulfate followed by polyacrylamide gel electrophoresis (SDS-PAGE) combined with the single-wavelength transmission method using a linear scan,phylogenetic analysis of the second internal transcribed spacer (ITS2)sequence data,structural analyses of the positional entropy and ITS2 base-pair probabilities,and a comparative analysis of the ITS2structures.Results:Based on our phylogenetic analysis,only 1 sequence (sinensis 6) was separated from the rest.This finding may indicate that most Dang-gui ITS2 sequences are conserved.The ITS2 sequence of this test sample shared 97.7% similarity with those of the Dang-gui clade.Dang-gui and Ou-dang-gui were identified as sister taxa,sharing 85.5% similarity at the sequence level.The two conserved overlapping structures were identified in the Dang-gui ITS2 structures.The other umbellifers did not possess these two conserved overlapping structures.There are two similar structures in Ou-dang-gui,but these structures did not have the highest probability and were not entropy-minimal structures.Thus,the two conserved overlapping structures may be markers for Dang-gui.Conclussion:The study may support the use of several simple and economical methods to identify the medicinal plant Dang-gui.Using all four methods,we were able to identify our test sample as Dang-gui,with an overall success rate of more than 177.7%.