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Single-stranded DNA with G-rich sequences can fold into secondary structures,G-quadruplexes,via intramolecular hydrogen-bonding interactions,which have attracted immense research attention as prospective targets for chemical intervention of biological functions among various noncanonical nucleic acid structures.1-2 We report here on a novel water-soluble G-quadruplex DNA fluorescent sensor based on the photoswitching of a spiropyran derivative (QNL-SP).