【摘 要】
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Objective: To observe the protective effects of Yangjing capsule on testicular damage of adult male mice induced by cyclophosphamide(CP) and the mechanism.METHODS: Fifty adult male mice were randomly
【机 构】
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Institute of Andrology,Nanjing University of Chinese Medicine,Nanjing,Jiangsu 210023,China
【出 处】
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中华中医药学会第十四次男科学术大会
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Objective: To observe the protective effects of Yangjing capsule on testicular damage of adult male mice induced by cyclophosphamide(CP) and the mechanism.METHODS: Fifty adult male mice were randomly categorised into five groups,10 in each group.For the first seven days,control group mice received intraperitoneal (IP) injection of saline solution once a day.The Cp group and low,middle,high dose of Yangjing capsule group mice were injected intraperitoneally with 50mg/kg Cp daily to induce testicular injury.For the next 30 days,control group and Cp group were administered saline solution orally,while low,middle,high dose of Yangjing capsule group received 630mg/kg,1260mg/kg,2520mg/kg Yangjing capsule suspension by oral gavage respectively.At the end of the treatment period,bodyweight and the weight of testis and epididymis,sperm count,sperm activity,the rate of sperm deformation were recorded,and the level of testosterone and Inhibin in serum were detected.Meanwhile the histopathological changes in testis were observed by light microscopy,damage to testicular tubules was determined.Spermatogenic cell apoptosis was examined by TUNEL.RESULT: Co-treatment with Yangjing capsule suspension significantly increased diminished bodyweight and the weight of testis and epididymis,sperm count,sperm activity and decreased the number of the abnormal sperms and damaged testicular tubules in mice treated with CP.The level of testosterone and Inhibin in serum in Yangjing capsule group had an increase compared to CP group and spermatogenic cell apoptosis were significantly decreased.CONCLUTION: Yangjing capsule can significantly improve the testicular damage induced by cyclophosphamide in mice.
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