Fangji huangqi decoction(FJHQ)is a compound Chinese medicine for treatment of chronic kidney disease which has been extensively used in clinical practice.Due to lack of appropriate methods,pharmacological mechanisms of FJHQ acting on CKD have not been fully elucidated.In this study,by incorporating network biology,bioinformatics and chemoinformatics methods,an integrated approach was proposed to systematically investigate and explain the pharmacological mechanism of FJHQ.In addition,we established a UUO model to validate the mechanisms of FJHQ action that were predicted in systems pharmacology study.The ADME/T model derived 198 active compounds from FJHQ and 468 putative targets related to various diseases.Then,the protein-protein interaction network of the putative targets of FJHQ and known CKD-related targets was constructed and 189 potential targets were identified by topological features.The ClueGo assay revealed that potential targets were most related to renal fibrosis and inflammation.Further experimental validation also demonstrated the therapeutic effect of FJHQ on UUO induced CKD rats and its regulatory effects on potential targets in integral animal experiment.