A simple,rapid and sensitive immunogold chromatographic strip test based on a monoclonal antibody was developed for the detection of melamine(MEL)residues in raw milk,milk products and animal feed.The limit of detection was estimated to be 0.05 μg/mL in raw milk,since the detection test line on the strip test completely disappeared at this concentration.The limit of detection was 2μg/mL(or 2μg/g)for milk drinks,yoghourt,condensed milk,cheese,and animal feed and 1 μg/g for milk powder.Sample pretreatment was simple and rapid,and the resuits can be obtained within 3-10 min.A parallel analysis of MEL in 52 blind raw milk samples conducted by gas chromatography-mass spectrometry showed comparable results to those obtained from the strip test.The results demonstrate that the developed method is suitable for the onsite determination of MEL residues in a large number of samples.