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目的:观察补中益气汤含药血清对转化生长因子-β1(TGFβ1)介导的人肺腺癌A549细胞上皮间质转化过程中波形蛋白(Vimentin)及谷胱甘肽-S-转移酶π(GST-π)表达的影响。方法:制备不同浓度的补中益气汤含药血清作用于TGF-β1诱导的A549细胞,实验分组为空白血清组,空白血清+TGF-β1组以及高、中、低剂量补中益气汤含药血清+TGF-β1组。采用免疫组化、Western blot和Real-time PCR法分别检测Vimentin及GST-π的蛋白和m RNA变化。结果:高、中、低剂量的补中益气汤含药血清均能使TGF-β1诱导的上皮间质化的A549细胞中Vimentin和GST-π蛋白表达下降,m RNA表达水平降低,其中高、中剂量组与空白血清组比,差异亦有统计学意义(P<0.05,P<0.01)。结论:补中益气汤含药血清能抑制TGF-β1介导的A549细胞上皮间质化中Vimentin及GST-π的表达,改善其耐药性。
OBJECTIVE: To observe the effect of Buzhong Yiqi decoction-containing serum on the expression of vimentin and glutathione-S-transferase in the process of epithelial-mesenchymal transition of human lung adenocarcinoma A549 cells mediated by transforming growth factor-β1 (TGFβ1) π (GST-π) expression. Methods: Different concentrations of Buzhong Yiqi decoction-containing serum were used to treat A549 cells induced by TGF-β1. The experimental group was divided into blank serum group, blank serum + TGF-β1 group and high, medium and low dose Buzhong Yiqi Decoction Drug-containing serum + TGF-β1 group. Immunohistochemistry, Western blot and Real-time PCR were used to detect the protein and mRNA expression of Vimentin and GST-π. Results: High, medium and low doses of Buzhong Yiqi Decoction serum could decrease the expression of Vimentin and GST-π protein and the mRNA expression of m RNA in TGF-β1-induced epithelial-mesenchymal A549 cells (P <0.05, P <0.01). There was also significant difference between middle dose group and blank serum group (P <0.05, P <0.01). Conclusion: Buzhong Yiqi decoction containing serum can inhibit the expression of Vimentin and GST-π in TGF-β1-mediated epithelial-mesenchyme of A549 cells and improve their drug resistance.