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The development of hydroxyapatite(HA)scaffolds for bone substitute are require an understanding of angiogenesis and vascular formation,which are necessary for bone formation and regeneration.There are few evidences show the ability of HA fiber scaffolds to support blood vessel formation;this is great importance because it related to the quality and dispersion of the blood vessel network.This work reports the influence of vascularization on 3D fiber scaffolds copper-substituted hydroxyapatite(Cu-HA)and the effect of microstructure surface on blood vessel formation and vascular guidance,using ex Ovo model,the egg chick embryo chorioallantoic membrane(CAM)assay.Two types of fiber scaffolds were fabricated;the first is pure HA without Cu++ions on the surface and microstructure surface process,and the second Cu-HA scaffolds were coated with a little amount of Cu++ions.Hydrothermal method are used to improve microstructure surface and ions release.The two types of scaffolds were implanted into chick egg for 4-8-14 days.After the periodic time,all samples were tested and analyzed with optical microscopic and SEM,before and after implantation.The results have divided into two part:The first result,Cu-HA fiber scaffold by its self-assembled changed the microstructure surface from smooth to roughness topography comparing with pure HA scaffolds.The second result,HA pure fiber scaffold did not influence angiogenesis,but Cu-HA fiber scaffolds affected the blood vessel formation and pores vessel guidance.That refer to the changed and improved in scaffold biological properties.The conclusion of this experiment shows that the Cu-HA fiber scaffolds with two factor,the amount of copper and new microstructure surface had affected the blood vessel formation and pore vessel guidance.The results contribute that is the Cu-HA fiber scaffolds bioactive materials,however,and the two-factor together improve the blood vessel formation on egg chick implantation.The new property is significant and gives the product ability to play the role on born substitute regeneration and healing.